The lab says fixation will cause the dyes to leak through the cells. I believe the assumption is usually that fixation may hurt mobile membranes. I did find a paper which describes fixation employing methanol or acetic acid, right after staining using a DCFDA spinoff. DCFDA is the reagent that https://donovanjmict.blazingblog.com/29417173/2-fdck-hcl-secrets